normal skin fibroblast cell bj  (ATCC)

Journal: Frontiers in Medicine

Article Title: Modifications in cellular viability, DNA damage and stress responses inflicted in cancer cells by copper-64 ions

doi: 10.3389/fmed.2023.1197846

Figure Lengend Snippet: Dose-effect analysis on MTS reduction by human normal BJ fibroblasts and by various human tumor cells (HT29 and HCT116 colon carcinoma, and DU145 prostate carcinoma) exposed to 64 Cu in the dose range of 2, 10, 20 and 40 MBq/mL for various time points: (A) 24 h; (B) 48 h; (C) 72 h. Results are presented as mean ± SEM for triplicate samples. 64 Cu effect was calculated as MTS reduction value (OD) for each sample of the triplicate divided by the mean value of MTS reduction in the non-treated controls.

Article Snippet: Three human tumors lines: HT29 (colon adenocarcinoma, ATCC HTB-38), HCT116 (colon carcinoma, ATCC CCL-247), DU145 (prostate carcinoma, ATCC HTB-81), and normal human skin fibroblast cell line BJ (ATCC CRL-2522) were cultivated according to the depositor’s proposed protocols (ATCC, American Tissue and Cell Collection) at 37°C and 5% CO 2 in DMEM culture medium (Gibco, ThermoFisher Scientific, Waltham, MA, US) supplemented with 10% FBS (Euroclone, Milan, Italy), and a stabilized Antibiotic Antimycotic Solution (100x) (Sigma, Saint Louis, MO, US) containing 10,000 units penicillin, 10 mg streptomycin and 25 μg Amphotericin B per mL.

Techniques:

Journal: Frontiers in Medicine

Article Title: Modifications in cellular viability, DNA damage and stress responses inflicted in cancer cells by copper-64 ions

doi: 10.3389/fmed.2023.1197846

Figure Lengend Snippet: Changes in the expression of stress genes triggered by the treatment of human normal BJ fibroblasts with 20 MBq/mL 64 Cu for 24 h. qRT-PCR results were presented as mean value of Fold Change (FC) ± SEM for 3 independent experiments. Only genes with significant expression changes (1.5 < FC < 0.7) were represented.

Article Snippet: Three human tumors lines: HT29 (colon adenocarcinoma, ATCC HTB-38), HCT116 (colon carcinoma, ATCC CCL-247), DU145 (prostate carcinoma, ATCC HTB-81), and normal human skin fibroblast cell line BJ (ATCC CRL-2522) were cultivated according to the depositor’s proposed protocols (ATCC, American Tissue and Cell Collection) at 37°C and 5% CO 2 in DMEM culture medium (Gibco, ThermoFisher Scientific, Waltham, MA, US) supplemented with 10% FBS (Euroclone, Milan, Italy), and a stabilized Antibiotic Antimycotic Solution (100x) (Sigma, Saint Louis, MO, US) containing 10,000 units penicillin, 10 mg streptomycin and 25 μg Amphotericin B per mL.

Techniques: Expressing, Quantitative RT-PCR